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Activated Reptilase Clot Retraction of Dog Platelet‐Rich Plasma: Differences with Human Platelet‐Rich Plasma
Author(s) -
Clerck F.,
Verheyen A.,
Vermylen J.,
Reneman R.
Publication year - 1975
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1975.tb01090.x
Subject(s) - platelet , chemistry , platelet rich plasma , clot retraction , platelet adhesion , inducer , adhesion , cytoplasm , human plasma , batroxobin , biophysics , clot formation , platelet aggregation , microbiology and biotechnology , biochemistry , immunology , fibrinogen , biology , chromatography , organic chemistry , gene
The retraction of dog platelet‐rich plasma (PRP) clotted with Reptilase in the presence of inducers and inhibitors of the platelet adhesion‐aggregation reaction was studied. In contrast to human cells, dog platelets fail to support retraction in an ADP‐Reptilase system. With Thrombofax as aggregation inducer, slight (without additional CaCl 2 ‐MgCl 2 ) to moderate (with additional CaCl 2 ‐MgCl 2 ) retraction occurs. In contrast to human samples, such retraction is inhibited by platelet release inhibitors. Electron‐microscopic examinations show that Thrombofax, but not ADP, induces the formation of large cytoplasmic protrusions from dog platelets. Such formation is inhibited by release‐inhibitors. The results of the study support the concept that platelet pseudopod formation, rather than the release reaction, is a prerequisite for clot retraction.