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Ultrastructural Study of Bone Marrow‐Derived Granulocytic Colonies in Diffusion Chambers in Rats
Author(s) -
BenIshay Zina
Publication year - 1975
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1975.tb01079.x
Subject(s) - ultrastructure , apposition , bone marrow , haematopoiesis , biology , pathology , fibrin , microbiology and biotechnology , anatomy , immunology , medicine , stem cell
An ultrastructural study of diffusion chamber granulocytic colonies is reported. The colonies were derived from normal rat bone marrow cells and the chambers were inserted into the peritoneal cavity of lethally irradiated rats. The colonies were processed en bloc for electron microscopy enabling observation of cellular relationship to be made. Ultrastructural analysis of colonies disclosed normal maturing cells at all stages of maturation, with the exception of occasional granulocytes of neutrophil type that presented narrow strips of chromatin (‘nuclear blebs’). The cells in colonies generally occurred in close apposition to one another and to occasional macrophages. This organization possibly enhanced transmission of factors of maturation. Advantages of diffusion chamber haematopoietic cultures are discussed; among the most important are the occurrence of fibroblasts originating from implanted marrow cells and the local formation of fibrin within the chambers.