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Technical Aspects of the Rosette Technique for Detecting Human Circulating B and T Lymphocytes Normal Values and Some Remarks on Null Lymphocytes
Author(s) -
Jønsson Vigco
Publication year - 1974
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1974.tb00282.x
Subject(s) - rosette (schizont appearance) , rosette formation , incubation
The effect of variations in several single steps in the rosette technique for detecting human circulating B, T and O cells is investigated in order to describe the optimal production of B and T rosettes. Variations in pH, duration of incubation, temperature, EAC cell production, age of EAC cells and definition of a rosette are investigated. Using the optimal rosette technique, normal values for 15 healthy men and 15 healthy women (age range 18–65 years) are found to be: B lymphocytes 27.5 % or 477/μ1, T lymphocytes 60.6 % or 1049/μl, O lymphocytes 11.9 % or 206/μl. It is concluded that variations in the analytical procedure may greatly affect the outcome. The existence of O cells is briefly discussed.