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Enrichement of PHA‐Responsive Lymphocytes in Chronic Lymphocytic Leukaemia after Removal of Ig‐Bearing Cells
Author(s) -
Blomgren Henric,
Anderson Birger,
Johansson Bo
Publication year - 1974
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1974.tb00281.x
Subject(s) - phytohaemagglutinin , in vitro , antibody , peripheral blood , immunology , surface immunoglobulin , biology , microbiology and biotechnology , chemistry , b cell , biochemistry
Lymphoid cells from the peripheral blood of subjects with chronic lymphocytic leukaemia (CLL) were found to be poorly stimulated with phytohaemagglutinin (PHA) in vitro. Approximately 90 % of the cells had membrane bound Ig molecules. Enrichement of the non‐Ig bearing cells by passage through bead columns coated with human Ig and rabbit anti‐human antibody resulted in an increment of the relative PHA‐responsiveness of the cells. The results indicate that the low PHA‐respon‐siveness of lymphoid cells from CLL‐patients is due to the fact that the normal PHA‐responsive lymphocytes are massively contaminated with leukaemic, unresponsive cells which bear surface Ig.