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Isolation and Characterization of an in vivo Thrombin‐Induced Anticoagulant Activity
Author(s) -
Hyde Elisabeth,
Wetmore Robert,
Gurewich Victor
Publication year - 1974
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1974.tb00245.x
Subject(s) - thrombin , in vivo , chemistry , in vitro , coagulation , clotting factor , vitamin k , vitamin , biochemistry , chromatography , pharmacology , platelet , medicine , biology , microbiology and biotechnology
An anticlotting activity present in rabbit blood following the administration of small amounts of thrombin is described. The substrate for this activity was found to be related to the vitamin‐K dependent clotting factors. The activity was labile in blood but relatively stable after it was isolated from plasma of thrombin treated animals. Oxy‐genation of blood was found to accelerate its inactivation. A method for the rapid chromatographic isolation of the activity from small amounts of plasma is described. The method is considered sufficiently practical to be utilized clinically. The anti‐clotting activity is considered similar to that which can be elaborated in vitro from thrombin activation of isolated prothrombin complex as previously described by others. A physiologic role for the activity is proposed.