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Complement Components on the Surface of Normal Human Lymphocytes
Author(s) -
Osther K.,
Dybkjaer E.
Publication year - 1974
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1974.tb00231.x
Subject(s) - antibody , complement (music) , immunofluorescence , immune system , surface immunoglobulin , peripheral blood , immunology , cytotoxicity , complement dependent cytotoxicity , complement system , biology , chemistry , microbiology and biotechnology , antibody dependent cell mediated cytotoxicity , b cell , monoclonal antibody , biochemistry , in vitro , complementation , gene , phenotype
The presence of complement components Clq, C4, C3 and C3 activator is demonstrated on human lymphocytes from peripheral blood of healthy subjects. The methods employed are double layer immunofluorescence technique and cytophoto‐metry. Furthermore, simultaneous detection of immunoglobulin and complement components has been possible. An amount of 10–25 % of the isolated lymphocytes showed presence of complement components, distributed in a manner comparable to immunoglobulins on the surface of the lymphocytes. The simultaneous double marking technique revealed an invariable co‐occurrence of immunoglobulins and complement components on the lymphocytes. This study may indicate that the cytotoxicity of the B lymphocytes resembles the cytotoxical actions of the humoral immune system.