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Granulopoiesis in Chronic Myeloid Leukaemia I. In vitro Cloning of Blood and Bone Marrow Cells in Agar Culture
Author(s) -
Moberg C.,
Olofsson T.,
Olsson I.
Publication year - 1974
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1974.tb00225.x
Subject(s) - granulopoiesis , bone marrow , peripheral blood mononuclear cell , chronic myeloid leukaemia , colony stimulating factor , immunology , myeloid , in vitro , biology , haematopoiesis , stem cell , microbiology and biotechnology , biochemistry
Peripheral blood and bone marrow cells from patients with chronic myeloid leukaemia were grown in agar culture. The number of colonies grown from the blood was 1,000–60,000 times greater than in normal controls. Some correlations was found between the concentration of leucocytes and colony forming cells of the blood. The autostimulation found in some cases might indicate increased production of the colony stimulating factor in chronic myeloid leukaemia. Mature granulocytes from both healthy and leukaemic donors inhibited to about the same degree colony formation from low density marrow cells of control donors also in the, presence of a feeder layer of mononuclear blood leucocytes. It is possible that a control mechanism of granulopoiesis involves feed back inhibition by substances produced by mature granulocytes. In a few cases of chronic myeloid leukaemia the colony forming cells seemed to be resistant to the inhibitory action of mature granulocytes.