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Human Platelet Aggregation by Thrombofax® An Electron‐Microscopic Study of the Sequence of Events
Author(s) -
Clerck F. de,
Borgers M.,
Vermylen J.,
Gaetano G.
Publication year - 1974
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1974.tb00187.x
Subject(s) - pseudopodia , serotonin , platelet , ultrastructure , chemistry , degranulation , biophysics , adhesion , platelet aggregation , microtubule , electron microscope , biochemistry , anatomy , microbiology and biotechnology , biology , cell , immunology , organic chemistry , receptor , physics , optics
The ultrastructural changes and the kinetics of 14 C‐serotonin release during the adhesion‐aggregation reaction of human platelets induced by Thrombofax® (Ortho Pharmaceuticals), a commercial cephalin prepared as an ether extract of acetone dried bovine brain, were analyzed. The initial phase of interaction of Thrombofax with platelets is characterized by the development of adhesion sites on normally shaped cells; thereafter, loss of discoid shape, pseudopod formation and inward movement of granules, surrounded by a circumferential bundle of microtubules, become apparent. Measurements of 14 C‐serotonin release show that these ultrastructural changes precede the release reaction. The later stages which are detectable turbidometrically and which only occur in stirred samples and in the absence of release inhibitors, are characterized mainly by large aggregate formation, platelet degranulation and strong release of 14 ‐C‐serotonin.