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Pattern of the Activator Inhibitor in Carcinoma and Renal Diseases
Author(s) -
Hedner U.,
Nilsson I. M.
Publication year - 1973
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1973.tb00150.x
Subject(s) - sephadex , urokinase , size exclusion chromatography , plasminogen activator , renal function , chronic renal insufficiency , chronic renal failure , lysis , carcinoma , medicine , chemistry , endocrinology , gastroenterology , biochemistry , enzyme
The pattern, after gel filtration of serum on Sephadex G‐200, of the inhibitors of the urokinase activation of plasminogen was studied in 20 patients with carcinoma, 12 with chronic renal insufficiency and 10 with acute renal insufficiency. The urokinase inhibitors were determined with the clot lysis method of Paraskevas et al (1962) and in the patients with carcinoma and those with chronic uraemia also with the caseinolytic method of Lauritsen (1968). The amount of inhibitors obtained in the high molecular weight fraction after gel filtration of sera from patients with carcinoma did not differ from normal, while that obtained in the low melecular fraction was significantly raised when assayed with both methods. After gel filtratinon of sera from the patients with chronic or acute renal failure the amount of inhibitors in each fraction was significantly increased when determined with the clot lysis method. But in the patiens with chronic renal failure no increase was found when measured with the caseinolytic method. In these patients the inhibitory effect on the activation of plasminogen is at least partly unspecific owing to accumulation of amino acids in the plasma in uraemia.