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The Influence of Fibrinogen Degradation Products on the Reptilase‐Time of Plasma
Author(s) -
Arnesen Harald,
Kierulf Peter,
Godal Hans Chr.
Publication year - 1973
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1973.tb00144.x
Subject(s) - fibrin , fibrinogen , chemistry , thrombin , reagent , batroxobin , coagulation , chromatography , biochemistry , polymer chemistry , immunology , platelet , organic chemistry , medicine
The Reptilase and thrombin clotting times of plasma containing increasing amounts of various FDP, and from patients on urokinase therapy, were equally prolonged. At the point of visible gelation, equal amounts of fibrin were formed with Reptilase and thrombin, as indicated by generation of N‐terminal glycine. Dialysis of the Reptilase reagent markedly increased the effect of FDP on the Reptilase time. The presence of glycine in the Reptilase reagent is suggested to counteract the influence of polymerization inhibitors, by lowering pH in the clotting mixture and by decreasing the solubility of fibrin/fibrinogen.