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Normal Red Cell Survival in the Rabbit
Author(s) -
Smith G. N.,
Mollison P. L.
Publication year - 1973
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1973.tb00115.x
Subject(s) - elution , red cell , life span , in vivo , cell , chemistry , chromatography , andrology , biology , medicine , biochemistry , genetics , evolutionary biology
Red cell survival in the rabbit was estimated using both 51 Cr to label cells in vitro and DF 32 P to label cells in vivo. The results suggest that, after about the first 7 days, there was no elution of DF 32 P from the red cells and that, accordingly, the rate of 51 Cr elution could be obtained by plotting the 51 Cr/DF 32 P ratio from day 7 onwards. If the assumption is made that the rate of Cr elution was the same between days 1 and 7 as it was after day 7, then observed Cr values from day 1 onwards can be corrected for elution and an estimate of true red cell survival obtained. Analysis of the resulting curves indicates that the initial slope is linear on an arithmetical scale for about the first 17 days, becoming curvilinear thereafter. It is concluded that the curvilinear slope of red cell survival in rabbits, hitherto ascribed to random red cell destruction, is more likely to be due to a rather wide scatter of red cell life‐span combined with superimposed elution of the red cell label. In the present series, mean red cell life‐span in the rabbit was estimated to be 50.4 days; as in previous series, the survival of the longest‐lived cells was found to be about 65–70 days.