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Electrophoresis of Blood Platelets and Erythrocytes: A Study in Healthy Persons and Patients with Haematological Diseases, Prosthetic Heart Valves, Virus Infections and Hyperlipaemia
Author(s) -
Grøttum Kjell A.
Publication year - 1973
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1973.tb00112.x
Subject(s) - platelet , saline , red cell , phosphate buffered saline , red blood cell , chemistry , platelet poor plasma , medicine , endocrinology , andrology , platelet rich plasma , chromatography
The mean electrophoretic mobility of normal human blood platelets in 301 samples from 260 healthy individuals was 1.08 μm/s/V/cm in citrated plasma and 0.84 and 1.05 μm/s/V/cm in 0.145 M saline (pH 7.3) and in sucrose/phosphate/saline buffer (pH 6.4), respectively. Normal red cell mobility in citrated plasma in 229 samples from 195 persons was 1.41 μm/s/V/cm, in 0.145 M saline and 0.0667 M phosphate buffer (pH 7.3) 1.09 and 1.28 μm/s/V/cm, respectively. The results of blood platelet and red cell electrophoresis in 90 patients with various diseases are reported. Severe abnormalities in platelet mobility were not found in any of the patients. In patients with von Willebrand's disease a slight increase in platelet mobility was observed. In 4 patients with haemolytic‐uraemic syndrome and 2 patients with microangiopathic haemolytic anaemia a reduced red cell mobility in citrated plasma and buffers was found. In a case of red cell polyagglutinability, two populations of erythrocytes were observed electrophoretically. About half of the red cells had a mobility of about 43 % of normal in citrated plasma.