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Perturbation of Generation Cycle of Human Leukaemic Blast Cells in vivo by Daunomycin
Author(s) -
Ernst Peter
Publication year - 1973
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1973.tb00091.x
Subject(s) - in vivo , mitotic index , in vitro , thymidine , dna synthesis , mitosis , precursor cell , cell cycle , cell , dna , chemistry , biology , cancer research , microbiology and biotechnology , biochemistry , genetics
The in vivo effect of daunomycin on the cell kinetics of leukaemic blast cells has been investigated in 4 patients with acute myeloblasts leukaemia and in 2 patients with acute lymphoblastic leukaemia by a method of combined autoradiography and microspectrophotometry. After a dose of about 1.5 mg daunomycin/kg bw a decrease in the mitotic index was observed, and in 4 cases also a depression of the in vitro 3 H‐thymidine labelling index was noticed. Microspectrophotometric analysis showed an accumulation of cells in G 2 phase and a depression of DNA synthesis. In 3 of the patients studied a rapid decline in the peripheral blast cell count was noticed. The data indicate that the effect of daunomycin on cell proliferation is a randomized cytocidal effect, superimposed on which there is a specific inhibitory action on the progression of cells through G 2 phase and a depression of DNA synthesis.