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Changes in Spleen Cellularity and Erythrocyte Accumulation Following Rauscher Virus Infection in the Mouse
Author(s) -
Markoe Arnold M.,
Okunewick James P.
Publication year - 1973
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1973.tb00072.x
Subject(s) - spleen , reticulocyte , virus , biology , friend virus , white blood cell , cell , red blood cell , immunology , andrology , medicine , rna , biochemistry , gene
The nature of the relationship of the spleen enlargement effected by Rauscher virus infection of SJL/J mice has been investigated with reference to cell proliferation in the spleen and to splenic red blood cell pooling and anaemia. The gross splenic nucleated cell content was found to increase linearly with spleen weight. The ratio of total spleen cells to nucleated spleen cells was identical at a value of 1.75 to 1 for both normal and virus‐infected mice even though in the latter some spleen sizes were as high as 30 times normal. On a per milligram basis, the average splenic accumulation of 59 Fe‐labelled normal red blood cells by normal mice was approximately twice as great as by 18‐day leukaemic mice, even though the leukaemic spleens contained a greater fraction of the injected label. Anaemia was seen to develop shortly after injection of the virus, reducing the haematocrit to values of less than 40 by two weeks. Compensatory increase in reticulocyte number did not occur until after the major fall in venous haematocrit had already been achieved. Peripheral white blood cell counts increased with time after virus injection and these correlated positively with spleen size, the relation being logarithmic.