Premium
Degradation of Fibrinogen in vitro: Demonstration of Several Antigenic Intermediates by Specific Anti‐D and Anti‐E Serum
Author(s) -
GORMSEN JOHS.,
FEDDERSEN C.,
CLEMMENSEN I.,
ANDERSEN R. B.
Publication year - 1972
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1972.tb00989.x
Subject(s) - immunoelectrophoresis , antigenicity , antigen , sephadex , chemistry , electrophoresis , thermolabile , agarose , fraction (chemistry) , immunodiffusion , biochemistry , chromatography , fibrinogen , precipitin , microbiology and biotechnology , biology , immunology , enzyme
Degradation of human fibrinogen by porcine plasmin was illustrated by agar and by crossed immunoelectrophoresis using anti‐fibrinogen and monospecific anti‐D and anti‐E serum. Within one minute D+E‐antigenic fragments appear under the actual experimental conditions, showing the position in the electrophoretic systems as the so‐called X‐fraction. Their ability to clot decreases gradually after further digestion and some appear to become heat stable. An E‐antigenic fraction appears shortly after the first D+E‐antigenic fractions, is placed in agar electrophoresis as the so‐called Y‐fraction, is heat stable and non‐clottable, and disappears gradually when no D+E‐antigenic fractions are left, as the final 24 h E‐fraction develops. It is easily distinguished from the latter in crossed immunoelectrophoresis and, due to its higher molecular weight, by Sephadex G 200 column chromatography. The first D‐antigenic fragments appear contemporary with the first E‐antigenic fragments. Early and intermediate D‐antigenic fractions exhibit electrophoretic mobilities in crossed agarose electrophoresis different from that of the late D‐fraction. After sustained digestion fragments with partial D‐antigenicity develop.