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Survival in vivo of Human Blood Platelets Frozen without Protective Additives
Author(s) -
LUNDBERG A.,
MURPHY S.
Publication year - 1972
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1972.tb00934.x
Subject(s) - platelet , in vivo , in vitro , tonicity , dehydration , andrology , chemistry , fresh frozen plasma , biochemistry , immunology , medicine , biology , microbiology and biotechnology
This study was done to verify observations in vitro (1) that the platelets' resistance to slow freezing is greater than the red cells' (2) that it is equal to their resistance to hypertonic stress; and (3) that loss of viability caused by these factors can be estimated by measuring loss of serotonin binding capacity in vitro. 12 autologous transfusions were performed of platelets that has been frozen and thawed in plasma without cryoprotective additive. After freezing to — 3° and — 4° C for approximately 10 min recovery in vivo was normal; freezing to — 5°, — 6° and — 7°C resulted in decreased recovery (68%, 59% and 41% of expected). These results agree with in vitro data and support the view that hypertonic dehydration is the main cause of cellular freezing injury. They do not mean that platelets can be stored under these conditions; after 24 hrs frozen to — 4° C no platelets circulated after transfusion.