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The Influence of Storage on ADP‐Induced Platelet Aggregation
Author(s) -
SIVERTSEN ULLA
Publication year - 1972
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1972.tb00933.x
Subject(s) - platelet , chemistry , incubation , platelet aggregation , saline , incubation period , dialysis , biochemistry , endocrinology , medicine
The aggregability of platelets stored in plasma at room‐temperature, pH 7.2–7.4, decreased approximately by a factor ten over a 24 hr period. Storage at pH 6.5 resulted in a more pronounced decrease. In some experiments this was reversible if the platelets were allowed to regenerate by incubation at pH 7.4 one or two hrs before measuring the aggregation. The procedure of concentrating platelets as well as storing the platelets as a concentrate, contributed to a decrease in aggregability. Saline as a medium for storage caused complete loss of aggregability within a few hours. K + , Ca 2+ and glucose was shown by dialysis experiments to be vital factors for maintaining platelet aggregability during storage.