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Effects of Compound 48/80 on Platelets: Structural Alterations and Inhibition of Aggregation
Author(s) -
TS'AO CHUNGHSIN,
GLAGOV SEYMOUR
Publication year - 1972
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1972.tb00915.x
Subject(s) - platelet , chemistry , thrombin , degranulation , histamine , biophysics , fibrin , platelet aggregation , serotonin , biochemistry , compound 48/80 , dense granule , pharmacology , immunology , biology , receptor
Compound 48/80 degranulates mast cells and release histamine and serotonin. Since platelet degranulation is an important aspect of platelet function, the effect of this compound on platelets and platelet aggregation were investigated by transmission and scanning electron microscopy. Low concentrations of 48/80 did not alter platelet ultrastructure but high concentrations suppressed platelet pseudopod formation and altered the surface connecting system. Low concentrations of 48/80 interfered with platelet aggregation by thrombin and collagen but not by ADP; platelets incubated with high concentrations of 48/80 did not aggregate when exposed to ADP or collagen but the addition of large quantities of thrombin overcame this inhibition and caused platelet aggregation as well as plasma clotting. The effect of thrombin on platelets exposed to 48/80 was not related to the presence of fibrin. The observations provide evidence that the platelet membrane plays an essential role in aggregation and that the mechanisms leading to platelet release and aggregation by various agents may be different.