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Phytohaemagglutinin Stimulation of Peripheral Lymphocytes in Children with Acute Lymphoblastic Leukaemia during Remission
Author(s) -
BAKKEREN J. A. J. M.,
VAAN G. A. M.,
SCHRETLEN E. D. A. M.
Publication year - 1972
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1972.tb00911.x
Subject(s) - phytohaemagglutinin , vincristine , prednisone , cyclophosphamide , methotrexate , medicine , peripheral blood , stimulation , immunology , amethopterin , lymphoblast , peripheral , chemotherapy , biology , cell culture , genetics
PHA response in vitro of peripheral blood lymphocytes from patients with ALL was determined during remission by 3 H‐thymidine uptake. Short courses of intensive treatment with rubidomycin, vincristine, and prednisone significantly diminished both the PHA response and the number of circulating lymphocytes. PHA stimulation restored itself rather quickly after the cytostatic treatment. Maintenance therapy with 6‐mercaptopurine, methotrexate, or cyclophosphamide did not cause a significant change in PHA response. The results are discussed in relation to the immunological status of the patients and the possibilities of active immunotherapy.