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Red Cell Acetylcholinesterase and Adenosine‐Triphospatase Activity in Patients with a Positive Antiglobulin Test
Author(s) -
Scott G. L.,
Rasbridge M. R.
Publication year - 1971
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1971.tb02671.x
Subject(s) - acetylcholinesterase , aché , haemolysis , in vivo , red cell , antibody , chemistry , cell , spherocytosis , hereditary spherocytosis , medicine , cell membrane , enzyme , endocrinology , red blood cell , autoantibody , immunology , biochemistry , biology , splenectomy , spleen , microbiology and biotechnology
Red cell AChE activity was measured in 38 patients with a positive direct antiglobulin test and low levels were found in some of these patients. When related to cell age, assessed by red cell creatine content, these values appeared even lower. Density separation of differently aged cell populations showed that the reduction in AChE was due principally to an abnormal decline with cell ageing. The type of antibody sensitizing the cell was relevant as low levels of AChE were found more frequently with IgG antibodies. The reduction of AChE probably reflected a membrane lesion as it was associated with spherocytosis and haemolysis although ATPase, another membrane enzyme, was affected to a lesser extent. These changes could not be reproduced in vitro suggesting that the influence of some in vivo mechanism was essential.