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LDH Isoenzymes in Extreme Density Human Platelet Populations
Author(s) -
Knudsen Finn Ursin
Publication year - 1971
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1971.tb01972.x
Subject(s) - platelet , isozyme , chemistry , centrifugation , mean platelet volume , chromatography , biochemistry , enzyme , biology , immunology
By discontinuous gradient centrifugation in two oily mixtures of n‐dibutyl phthalate and Apiezon A oil of an accurately fixed specific gravity (1.0270 and 1.0405), platelets from 16 normal platelet pools were separated into extreme density platelet populations (large‐heavy and small‐light platelets) which were studied against unseparated control platelets for platelet volume and LDH isoenzymes. By the microhaematocrit method the mean platelet volume per platelet in large‐heavy, small‐light, and control populations was 11.6 μ 3 , 5.7 μ 3 , and 7.3 μ 3 . LDH isoenzyme determination, using disc polyacrylamide electrophoresis, revealed identical isoenzyme patterns in large‐heavy, small‐light, and control platelets. When expressed by the logarithm of the H/M ratio, the mean values for the three groups were 0.16, 0.15, and 0.15. Thus, the study adduced no evidence of a correlation between human platelet isoenzyme parameters and platelet volume.