Premium
Platelet Aggregation in Washed Suspensions
Author(s) -
Cronberg S.,
Caen J. P.
Publication year - 1971
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1971.tb01967.x
Subject(s) - platelet , chemistry , platelet aggregation , magnesium , calcium , fibrinogen , tris , adenosine diphosphate , chromatography , biophysics , biochemistry , medicine , organic chemistry , biology
Platelets were prepared from blood taken in EDTA. They were washed twice and resuspended in Tris buffer. To preserve their aggregability, they had to be kept at a temperature of 0–4° C and at a pH of 7.4. Because of a small content of spontaneously released ADP, magnesium and calcium alone induced some aggregation, especially at 23° C, and this was potentiated by human fibrinogen. Calcium produced more rapid aggregation than magnesium. Addition of ADP, adrenaline, or collagen induced marked aggregation. The method used was found useful in the investigation of platelet function, particularly in cases where plasma changes otherwise disturbed the aggregation.