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Splenic Destruction of Newly‐Formed Red Blood Cells and Shortened Erythrocyte Survival in Mice with Congenital Microcytosis
Author(s) -
Landaw Stephen Arthur,
Russell Elizabeth S.,
Bernstein Seldon E.
Publication year - 1970
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1970.tb01940.x
Subject(s) - microcytosis , haemolysis , erythropoiesis , hemolysis , reticulocyte , biology , red blood cell , andrology , strain (injury) , splenectomy , medicine , cytolysis , immunology , endocrinology , spleen , microbiology and biotechnology , anemia , iron deficiency , biochemistry , gene , anatomy , in vitro , messenger rna , cytotoxic t cell
Red blood cell survival and patterns of erythropoiesis‐associated haem destruction were studied in mice with microcytosis ( mklmk ) of the MK/Re and SEC/1Re strains, and their normal controls (+/+ and mk/+ ) using the endogenous production of 14 CO following glycine‐2‐ 14 C injection. In affected homozygotes of both strains, there was evidence for splenic destruction of reticulocytes and increased random haemolysis, with higher rates of random haemolysis in affected mice of the SEC/1Re strain. Alterations in the ‘early labelled peak’ of the mk/mk mice of the SEC/IRe strain suggested the presence of a small component of ‘ineffective erythropoiesis’ which was not seen in affected mice of the MK/Re strain. The ‘ineffective erythropoiesis’ and splenic reticulocyte destruction were abolished by prior splenectomy, but the rate of random haemolysis was only minimally lessened and splenectomized mice remained anaemic. These results offer direct insight into RBC survival patterns in genetically determined haemolytic anaemias, and document results of the interaction of a single gene defect ( mk ) with the rest of the genome.