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ADP‐Induced Aggregation of Washed Platelets Effect of Platelet and Plasma Fibrinogen
Author(s) -
Solum Nils Olav
Publication year - 1970
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1970.tb01894.x
Subject(s) - platelet , fibrinogen , apyrase , chemistry , biochemistry , platelet aggregation , cofactor , medicine , enzyme
ADP‐induced aggregation of washed human and porcine platelets has been studied. Plasma from a patient genetically deficient in fibrinogen lacked ADP‐cofactor activity. Apyrase totally inhibited the small platelet aggregation observed after addition of fibrinogen to washed platelets. Lysates of washed porcine platelets contained 1.34 mg/10 9 platelets of protein in the soluble form and 0.44 mg/10 9 platelets as insoluble protein. Platelet fibrinogen in soluble fraction was 0.16 mg/10 9 platelets. Partly purified porcine platelet fibrinogen showed cofactor activity for ADP‐induced aggregation of washed porcine platelets, but compared to plasma fibrinogen a higher concentration of the platelet fibrinogen was needed to obtain the same effect.