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Subbands of Lactate Dehydrogenase Isoenzymes in Human Thrombocytes
Author(s) -
Knudsen F. Ursin,
Gormsen Johs.
Publication year - 1970
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1970.tb01875.x
Subject(s) - lactate dehydrogenase , isozyme , electrophoresis , acrylamide , platelet , chemistry , polyacrylamide gel electrophoresis , microbiology and biotechnology , l lactate dehydrogenase , dehydrogenase , biochemistry , enzyme , biology , immunology , polymer , organic chemistry , copolymer
Isoenzymes of lactate dehydrogenase (LDH) from human platelets exhibit formation of subbands when examined by disc acrylamide electrophoresis using a 13% acrylamide in separating gel with a running temperature within the column of 45° C. at pH 8.9. One subband is found close to LDH 1, two bands close to LDH 2, all exhibiting a slower electrophoretic mobility than the main band. Three bands are seen in the LDH 3 area. No subbands formation is seen in the LDH 4 area. According to macroscopic evaluation identical parameters are seen whether the platelets are taken from normal controls or from patients suffering from diseases combined with a change in H/M ratio and/or myeloproliferative disorders.