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The Mode of Expressing the Content of Intracellular Components of Human Erythrocytes with Special Reference to Adenine Nucleotides
Author(s) -
Hjelm Magnus
Publication year - 1969
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1969.tb01804.x
Subject(s) - intracellular , nucleotide , biochemistry , mole , cell , adenine nucleotide , biology , chemistry , gene
No general agreement has been reached so far for the mode of expressing the content of intracellular components in human erythrocytes. The validity of the measures (1) moles per cell, (2) moles per cell volume and (3) moles per intracellular haemoglobin has been evaluated from a theoretical point of view in comparisons of different cell populations and in kinetic considerations. It was found that none of the expressions were valid if the intracellular concentration of water soluble components is concerned. The use of the expression (4) moles per liter of apparent cell water calculated from (2) and MCHC appears to be as a better approximation of the intracellular concentration of the components concerned. The consequences of these theoretical considerations when evaluating biochemical investigations on human erythrocytes, are exemplified by determinations of adenine nucleotides (ATP, ADP) in erythrocyte populations of differing mean age from patients with non‐spherocytic haemolytic anaemia and thalassaemia minor, and from healthy individuals. It can be shown that the conclusion to be drawn from the determinations are highly dependent on the mode of calculating the result.