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Studies of Platelet Adhesiveness with Reference to Platelet Age
Author(s) -
Rolovic Zoran,
Baldini Mario G.
Publication year - 1969
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1969.tb01797.x
Subject(s) - platelet , adhesive , in vitro , platelet adhesiveness , chemistry , adhesion , platelet aggregation , andrology , immunology , medicine , biochemistry , organic chemistry , layer (electronics)
In this study, autologous human platelets labelled with 31 Cr were infused in healthy volunteers and their adhesiveness to glass was repeatedly measured during their survival in the circulation using the Salzman's technique. 51 Cr‐specific activity was determined seperately for the ‘adhesive’ and ‘non‐adhesive’ platelets and expressed as a ratio of the two values. This ratio was found to be approximately 1 in all the conditions studied. The results indicated: (1) that platelet labelling in vitro with 51 Cr did not alter platelet adhesiveness to glass, and (2) that aging of the platelets in the circulation was not associated with a change in their adhesiveness. Other experiments were done using platelets which had been damaged by a 24hour storage at 4°C. before infusion. The results demonstrated that although survival of these platelets was drastically reduced, their adhesive property to glass remained unchanged. It was concluded that a large ‘reservoir’ of adhesive property must exist in the platelets and that this is independent of the damage produced by cell aging in the circulation and by storage in vitro at 4°C.