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DNA Synthesis Time and Related Parameters in Erythroid Cell Precursors of Rats
Author(s) -
Monette Francis C.,
LoBue Joseph,
Chan PoCheun,
Gordon Albert S.
Publication year - 1968
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1968.tb01756.x
Subject(s) - labelling , emulsion , dna synthesis , mitosis , dna , prophase , cell cycle , chemistry , flow cytometry , cell , microbiology and biotechnology , biology , biochemistry , meiosis , gene
The double‐labelling, two‐emulsion autoradiographic technique was used to study DNA synthesis time and related nuclear sub‐cycle durations of erythroid cell precursors in three week‐old rats. As employed in this short‐term investigation, the double‐labelling technique permitted direct estimates of DNA synthesis time to be made independently of labelling indices from the flow of two populations of labelled cells through the S‐phase of the nuclear cycle. The application of a second autoradiographic emulsion over single‐emulsion preparations made possible a more objective distinction between the two labels than can be done with single emulsion preparations. The following estimates were obtained from counts made on nine rats and a total of 45,000 cells: DNA synthesis time, 7.0 ± 0.5 hours; mitotic time (not including early prophase), 20 ± 3 minutes; average turnover time, 19.0 ± 1.8 hours.