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Carbohydrates in Erythrocyte Stroma: I. Concentration of Hexoses and Hexosamines in acid Hydrolysates of Human, Bovine, Pig and Horse Erythrocyte Stroma
Author(s) -
Relander Arvo
Publication year - 1968
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1968.tb01752.x
Subject(s) - stroma , hexosamines , hexose , hydrolysate , biochemistry , chemistry , chromatography , hydrolysis , chloroplast stroma , galactose , biology , enzyme , glucosamine , immunology , immunohistochemistry , thylakoid , chloroplast , gene
The optimal conditions for the hydrolysis of hexose and hexosamine components in the mucosaccharide fraction of red cell stroma were found to be 2 N HCl at 92° C. for 3 hours. The hydrolysate of stroma was converted by concentration, neutralization and ion exchange technique to a form in which the hexose and hexosamine components could be assertained. By determining the recovery and standard deviation the methods were found reproduceable. The normal values of total aldohexose, galactose, glucose and hexosamine concentrations were determined in stroma in about 20 cases of each of the following species: human, bovine, pig, and horse. The stroma was divided into two parts with a chloro‐form‐methanol mixture (2:1) and the hexose and hexosamine concentrations in each part were determined. Pig stroma, contrary to that of the other species, had markedly more hexoses in the lipid fraction than in the stroma residue fraction. The content of glucose in both fractions was the same in human stroma, but there were more hexoses and hexosamines in the stroma residue fraction. The hexosamine content in the lipid extract fraction of horse and human stroma was very low.