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Photometric Quantitation of Haemagglutination
Author(s) -
Dybkjaer E.
Publication year - 1967
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1967.tb01648.x
Subject(s) - hemagglutination , agglutination (biology) , titration , chromatography , dilution , hemagglutination tests , chemistry , sedimentation , antibody , serology , serial dilution , biology , physics , immunology , medicine , pathology , inorganic chemistry , paleontology , alternative medicine , sediment , thermodynamics
A modified technique for photometric quantitation of haemagglutination was employed in experiments with anti‐A test sera and Arcell suspensions. The agglutinates and the free cells were separated by simple sedimentation. As an absolute separation was impossible in this way, no exact estimation of percentage agglutination could be made. But an estimation of the antibody dilution which removes 50 per cent of the erythrocytes by agglutination and accelerated sedimentation was a far more accurate value for the strength of the antibody than the serological titration commonly used.
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