Premium
An Improved Method for Quantitative Estimation of Nucleated Cells in The Bone‐Marrow of Guinea Pigs by Using Liver Extract
Author(s) -
MäkitaloBäckström Ulla
Publication year - 1967
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1967.tb01634.x
Subject(s) - bone marrow , liver cell , cell , mitosis , chemistry , hepatic stellate cell , cell division , suspension culture , suspension (topology) , biology , chromatography , cell culture , pathology , biochemistry , immunology , medicine , endocrinology , microbiology and biotechnology , genetics , mathematics , homotopy , pure mathematics
Total counts of nucleated cells per μl bone‐marrow were determined in suspensions containing cell‐free liver extract and in suspensions without liver extract. The presence of liver extract caused a significantly higher cell count which increased with increasing amount of liver extract. The differences were not an effect of dilution nor were they caused by mitosis. No attempt was made to determine the optimal proportions of liver extract to suspension of cells, or the active component of liver extract. Bacteria or their products seemed not to be the possible cause of the increase in cell count. No formation of cell aggregates of importance was demonstrated in 20 minutes. It thus seems that the differences in the total count of cells is due to splitting of cell aggregates by a factor in liver extract. By adding cell‐free liver extract it thus seems possible to split aggregates of cells, and obtain a more correct count of cells in suspensions of bone‐marrow.