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Substitution of Plasma in Culture Medium with Dextran, Glucose or Albumin for in Vitro Studies of Lymphocytes
Author(s) -
Bergman Britt,
Börjeson Jan,
Löw Bengt,
Nordén ÅKe
Publication year - 1967
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1967.tb01617.x
Subject(s) - phytohaemagglutinin , in vitro , albumin , dextran , saline , chemistry , bovine serum albumin , nucleic acid , thymidine , biochemistry , chromatography , biology , endocrinology
Substitution of 6 per cent dextran solution in saline or 5.5 per cent glucose solution or 7 per cent human plasma albumin in saline for autologous plasma in the culture medium was tested by determination of 3 H‐thymidine incorporation in the nucleic acid fraction of lymphocytes cultured in vitro with phytohaemagglutinin or tuberculine. The different substitutes provided a medium which permitted uptake of 3H‐thymidine in the same range as autologous plasma.