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Reversible Agglutination of Trypsinised Red Cells by Normal Human Sera
Author(s) -
Mellbye Ove J.
Publication year - 1967
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1967.tb01611.x
Subject(s) - histidine , agglutination (biology) , trypsin , chemistry , biochemistry , microbiology and biotechnology , biology , immunology , antibody , amino acid , enzyme
The agglutination of trypsinised red cells by normal sera is reversed by a factor which is dialysable, thermostable, and present in normal human sera and urines. Among a series of substances with these properties, only histidine had reversing effect. Addition of histidine to dialysed serum restored the reversing ability. Like the reversor in serum, histidine did not affect the agglutination of cells modified by agents other than trypsin. It is concluded that the reversor in normal serum is identical with histidine. However, since the effect of histidine alone was somewhat weaker than that of normal serum, its effect may partly depend on additional unidentified serum factors.