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Separation of Lymphocyte‐Stimulating and Agglutinating Activities in Phytohaemagglutinin (PHA) from Phaseolus Vulgaris
Author(s) -
Weber Theodor,
Nordman Clas T.,
Gräsbeck Ralph
Publication year - 1967
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1967.tb01601.x
Subject(s) - phytohaemagglutinin , phaseolus , sephadex , lymphocyte , fractionation , chromatography , chemistry , phytohemagglutinins , biology , biochemistry , immunology , lymphocyte activation , botany , immune system , t cell , enzyme
A method for the fractionation of phytohaemagglutinin (PHA) extracted from Phaseolus vulgaris by chromatography on SE‐Sephadex C‐50 is described. The two lymphocyte‐stimulating substances obtained differ from each other in their chromatographic properties and their ability to agglutinate human blood cells. The procedure offers a simple method for the separation of lymphocyte‐stimulating and agglutinating substances in PHA.