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A Simple Procedure for the Determination of DNA Synthesis in Cultured Lymphocytes
Author(s) -
Börjeson Jan,
Gardell Sven,
Nordén ÅKe
Publication year - 1966
Publication title -
scandinavian journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0036-553X
DOI - 10.1111/j.1600-0609.1966.tb01437.x
Subject(s) - nucleic acid , papain , liquid scintillation counting , thymidine , chemistry , chromatography , dna , ammonium , dna synthesis , biochemistry , organic chemistry , enzyme
A simple method for the determination of the incorporation of H 3 ‐thymidine in cultured lymphocytes is described. The cells are broken up by treatment with papain at 65° C. Nucleic acids are then precipitated with a quaternary ammonium compound. The water insoluble complex is dissolved in methanol and the radioactivity determined in a liquid scintillation spectrometer. The method offers special advantage when large number of cultures have to be studied.