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Polymerase chain reaction‐denaturing gradient gel electrophoresis (PCR/DGGE)‐based detection of clonal T‐cell receptor γ gene rearrangements in paraffin‐embedded cutaneous biopsies in cutaneous T‐cell lymphoproliferative diseases *
Author(s) -
Andersen William K.,
Li Ning,
Bhawan Jag
Publication year - 1999
Publication title -
journal of cutaneous pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.597
H-Index - 75
eISSN - 1600-0560
pISSN - 0303-6987
DOI - 10.1111/j.1600-0560.1999.tb01825.x
Subject(s) - polymerase chain reaction , clone (java method) , gene rearrangement , temperature gradient gel electrophoresis , biology , t cell receptor , microbiology and biotechnology , cutaneous t cell lymphoma , lymphoma , pathology , gene , lymphoproliferative disorders , t cell , mycosis fungoides , immunology , medicine , genetics , immune system , 16s ribosomal rna
Polymerase chain reaction (PCR)‐based amplification of T‐cell receptor (TCR)‐γ genes is a novel technique that can detect a clone of T cells comprising less than 1% of the total T cells in a lymphoid infiltrate 1 . Besides greater sensitivity than Southern blotting, this technique can be performed with smaller quantities of lower molecular weight genomic DNA as template. We retrospectively analyzed 12 paraffin‐embedded biopsies of cutaneous T‐cell lymphoma (CTCL), 1 case suspicious for CTCL, 1 case of granulomatous slack skin, and 8 cases of inflammatory skin diseases to determine if PCR‐denaturing gadient gel electrophoresis (PCR‐DGGE) analysis can detect TCR‐γ gene rearrangements on paraffin‐embedded specimens. we were able to amplify Bγ1‐8 TCR sequences in each case and detected a dominant clone in 9 of 12 cases of CTCL and in granulomatous slack skin. We analyzed Vγ9 sequences in 9 cases of CTCL and detected a dominant clone in 4 cases. This study demonstrates that PCR‐DGGE can easiy by applied retrospectively to cutancous biopsies of lymphoproliferative diseases when fresh itssue is not available.