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The epidermotropic mycosis fungoides associated α1β1 integrin (VLA‐1, CD49a/CD29) is primarily a collagen IV receptor on malignant T cells
Author(s) -
Bank IIan,
Rapman Edward,
Shapiro Raisa,
Schiby Ginette,
Goldberg Iris,
Barzilai Aviv,
Trau Henry,
Gur Hanan
Publication year - 1999
Publication title -
journal of cutaneous pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.597
H-Index - 75
eISSN - 1600-0560
pISSN - 0303-6987
DOI - 10.1111/j.1600-0560.1999.tb01804.x
Subject(s) - mycosis fungoides , jurkat cells , integrin , extracellular matrix , microbiology and biotechnology , pathology , monoclonal antibody , cell adhesion molecule , receptor , cancer research , chemistry , lymphoma , t cell , biology , immunology , antibody , medicine , immune system
Several of the βa integrin receptors [very late antigen (VLA) molecules] for extracellular matrix (ECM) proteins are expressed by malignant T cells in cutaneous T‐cell lymphoma (CTCL). We evaluated the function of VLA‐1. a β1 integral specifically expressed in epidermotropic mycosis fungoides (MF), in CD4+ leukemic T cells (Jurkat line). We found that Jurkat cells adhere significantly to collagens only after their activation with phorbol 12‐myristate 13‐acetate (PMA). However, the adhesion to collagen IV (but not to collagen I) of Jurkat cells selected for expressing increased levels of VLA‐1 (with unchanged levels of VLA‐2, the second collagen integrin receptor) was significantly enhanced relative to that of “VLA‐1 low” cells. Monoclonal antibody (mAb) 1B3.1, directed against the collagen binding domain of VLA‐1, inhibited adhesion to collagen IV and to collagen I by 36.67%±5.25% and 18%±4.32%, respectively (p<0.05), whereas the inhibition by anti‐VLA‐2 mAb PIE6 was comparable on both collagens (25%±7.48% and 36.3%±0.94%, respectively; p<0.09). Immunohistochemical studies of skin biopsies from 10 untreated MF patients showed that in all cases at least 10% of the lymphocytes residing in the epidermis are VLA‐1+VLA‐2‐. While not directly applicable to MF, the demonstrated functions of VLA‐1 in leukemic Jurkat cells, together with its expression in MF skin, suggest a role for VLA‐1 integrins in epidermotropism in a small proportion of leukemic MF cells.