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DNA determination of human hair bulbs in normal and androgenetic alopecia
Author(s) -
Villena A.,
Alcaraz M.V.,
Vargas I. Pérez
Publication year - 1994
Publication title -
journal of cutaneous pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.597
H-Index - 75
eISSN - 1600-0560
pISSN - 0303-6987
DOI - 10.1111/j.1600-0560.1994.tb00709.x
Subject(s) - pathology , dna , dermatology , medicine , biology , genetics
We carried out a cytophotometric study of the DNA content of matrix cells from normal hair follicles and those affected by androgenetic alopecia (early and established baldness), using the Feulgen technique. We thereby obtained a 2C reference value from lymphocyte nuclei from normal skin, from which we established a 2C–4C and 4C DNA content. The results obtained showed 71.75% of matrix cells in normal scalp to have a 2C content, while the remaining 28.25% was distributed between the values of 2C–4C and 4C. However, in early baldness, the number of 2C cells dropped to 48%, with a notable increase in cell populations with a 2C–4C and 4C content, which varied from 29.45% and 22.55% respectively. Finally, in established baldness, the 2C values decreased to as low as 29.56%, with the percentage of cells with a 2C–4C content reaching 38.71% and those of 4C, 31.73%.