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Aneuploidy in atypical fibroxanthoma: DNA content quantification of 10 cases by image analysis
Author(s) -
Michie B.A.,
Reid R.P.,
Fallowfield M.E
Publication year - 1994
Publication title -
journal of cutaneous pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.597
H-Index - 75
eISSN - 1600-0560
pISSN - 0303-6987
DOI - 10.1111/j.1600-0560.1994.tb00280.x
Subject(s) - atypical fibroxanthoma , pathology , aneuploidy , lesion , cytology , ploidy , biology , feulgen stain , dna , immunohistochemistry , staining , medicine , chromosome , genetics , gene
It has recently been reported that atypical fibroxanthoma (AFX) is a predominantly diploid lesion in contrast to malignant fibrous hystiocytoma (MFH) which is usually aneuploid. To test this hypothesis, DNA content quantification was undertaken on Feulgen‐stained cytology and tissue section preparations from 10 cases of AFX by image analysis. The large‐atypical cells which characterize AFX were aneuploid in each case. Smaller spindle‐shaped cells found in this lesion were diploid. The results suggest that AFX is indistinguishable from MFH by DNA content estimation and highlight an advantage of image analysis over (low cytometry.