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Alpha‐6 (CD 49f) integrin expression in genetic and acquired bullous skin diseases
Author(s) -
Kanitakis J.,
Zambruno G.,
Vassileva S.,
Giannetti A.,
Thivolet J.
Publication year - 1992
Publication title -
journal of cutaneous pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.597
H-Index - 75
eISSN - 1600-0560
pISSN - 0303-6987
DOI - 10.1111/j.1600-0560.1992.tb00609.x
Subject(s) - bullous pemphigoid , pathology , hemidesmosome , skin biopsy , antigen , immunostaining , immunofluorescence , immunohistochemistry , dispase , antibody , medicine , biopsy , microbiology and biotechnology , basement membrane , biology , immunology , biochemistry , collagenase , enzyme
Bullous pemphigoid (BP) antigen and α6 integrin are hemidesmosome‐associated glycoproteins of basal keratinocytes. In this work, the immunoreactivity of antibodies to BP and to α6 in salt‐ or dispase‐split human skin, and in 46 biopsy specimens of various genetic and autoimmune bullous dermatoses taken from various body sites, was studied by double‐labeling immunofluorescence. In all specimens, both antigens localized at the same side of the blisters observed, i.e. the roof of the bulla in cases with a junctional or hemolytic cleavage, or the floor of the blister in those with intraepidermal cleavage. Immunostaining for α6 was strong and present in all specimens studied, whereas the one obtained with the BP serum was absent from some specimens. These results show that the BP antigen and the α6 integrin colocalize at the level of cleavage in bullous diseases; however, the more consistent and reproducible reactivity obtained with the anti‐α6 antibody suggests that this should be preferentially used in the immunohistochemical investigation of bullous dermatoses.