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Ultrastructural localization of HMB‐45 binding sites
Author(s) -
SchaumburgLever Gundula,
Metzler Gisela,
Kaiserling Edwin
Publication year - 1991
Publication title -
journal of cutaneous pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.597
H-Index - 75
eISSN - 1600-0560
pISSN - 0303-6987
DOI - 10.1111/j.1600-0560.1991.tb01380.x
Subject(s) - melanosome , ultrastructure , pathology , melanoma , hmb 45 , melanin , stage (stratigraphy) , biology , chemistry , anatomy , immunohistochemistry , medicine , cancer research , biochemistry , paleontology
Three malignant melanomas, two melanoma metastases, two junctional dysplastic nevi, and normal skin were embedded in Lowicryl. Ultrathin sections were incubated with HMB‐45 and a gold‐labeled anti‐mouse antibody. Gold particles indicating the presence of HMB‐45 were found in melanosomes Stage 1 and 2 and in the non‐melanized portion of melanosomes Stage 3. Melanosomes Stage 4 and melanosome complexes in keratino‐cytes, as well as in melanophages, were consistently negative. No specific labelling with HMB‐45 was seen in eccrine glands of normal skin.