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A replica technique for the evaluation of human skin by scanning electron microscopy
Author(s) -
Ryan R. L.,
Hing S. A. O.,
Theiler R. F.
Publication year - 1983
Publication title -
journal of cutaneous pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.597
H-Index - 75
eISSN - 1600-0560
pISSN - 0303-6987
DOI - 10.1111/j.1600-0560.1983.tb01492.x
Subject(s) - stratum corneum , replica , replication (statistics) , human skin , scanning electron microscope , biomedical engineering , stratum , pathology , materials science , biology , medicine , virology , art , paleontology , genetics , composite material , visual arts
A detailed reproducible procedure has been developed for the replication of human skin for scanning electron‐microscopic evaluation. Utilizing a test surface, a number of negative and positive materials were evaluated for consistence and clarity of replication. Optimal replication parameters were determined and the method was successfully applied to the evaluation of human skin in vivo. Furthermore, a method was established which permitted repetitive examination of selected skin sites. Experimental results suggest that this replicating procedure can be used to monitor changes in the stratum corneum overtime, and extend the range of stratum corneum magnifications.