Premium
Plastic embedding of cutaneous specimens: an important diagnostic aid in mycosis fungoides
Author(s) -
Barr Ronald J.,
Valdes Emilita R.,
Buck Janis E.
Publication year - 1982
Publication title -
journal of cutaneous pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.597
H-Index - 75
eISSN - 1600-0560
pISSN - 0303-6987
DOI - 10.1111/j.1600-0560.1982.tb01037.x
Subject(s) - mycosis fungoides , immunoperoxidase , araldite , pathology , mycosis , medicine , dermatology , lymphoma , chemistry , surgery , adhesive , layer (electronics) , antibody , immunology , monoclonal antibody , organic chemistry
A technique for primary plastic embedding for light microscopy was utilized in evaluating plaque lesions in four patients with mycosis fungoides and compared to tissues from the same patients embedded in paraffin. Standard 3 mm punch biopsies were utilized. Specimens were fixed in B‐5 and embedded in araldite epoxy resin. In specimens from all four patients, the cytological details of the mycosis cells were superior in the plastic embedded tissues. This practical and relatively inexpensive method may prove to be a sensitive indicator of mycosis cells in early lesions. This technique also permits the use of a variety of special stains, immunoperoxidase and electron microscopic analyses.