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T cell nature of exocytic and dermal lymphoid cells in atrophic parapsoriasis demonstrated by monoclonal Leu 1 and affinity isolated antibodies
Author(s) -
Mcmillan Euan M.,
Wasik Rose,
Martin Douglas,
Everett Mark Allen
Publication year - 1981
Publication title -
journal of cutaneous pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.597
H-Index - 75
eISSN - 1600-0560
pISSN - 0303-6987
DOI - 10.1111/j.1600-0560.1981.tb01021.x
Subject(s) - immunoperoxidase , monoclonal antibody , pathology , antibody , histopathology , cell , immunohistochemistry , primary and secondary antibodies , monoclonal , biology , immunology , medicine , biochemistry
Tissues from atrophic large plaque parapsoriasis were examined using the indirect immunoperoxidase technique with a monoclonal T cell antibody as primary antibody, and affinity isolated peroxidase conjugated goat anti‐mouse IgG as second stage antibody. The “T” cell nature of the dermal infiltrate and of single exocytic epidermal lymphocytes was demonstrated. The results obtained suggest that the method utilized will be useful for the demonstration of lymphoid cell subpopulations in a variety of cutaneous disorders in which a lymphocytic infiltrate forms a significant component of the histopathology observed. This technique also has the potential of providing information on the topographic localization and differentiation of lymphocytes within the various levels of the skin. Certain technical manipulations which may result in an improvement in the quality of results obtained are also discussed.