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Immunoelectron Microscopy of Skin in Lupus Erythematosus
Author(s) -
PEHAMBERGER H.,
KONRAD K.,
HOLUBAR K.
Publication year - 1978
Publication title -
journal of cutaneous pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.597
H-Index - 75
eISSN - 1600-0560
pISSN - 0303-6987
DOI - 10.1111/j.1600-0560.1978.tb00961.x
Subject(s) - dermis , lamina densa , basal lamina , immunoelectron microscopy , pathology , antibody , lamina lucida , immunoglobulin g , ultrastructure , biology , chemistry , medicine , immunology , immunohistochemistry
A multistep immunocytochemical method, utilizing horseradish peroxidase as an immunological bound marker enzyme, was employed to demonstrate in vivo fixed immunoglobulins (IgG, IgM) in the skin of three patients with CDLE and three patients with SLE. The immunoglobulin deposits were confined to the uppermost strata of the dermis, i.e. the area just below the basal lamina. Small amounts of immunoglobulin were visible within the lamina lucida and the basal lamina. Only quantitative differences were observed between deposits of immunoglobulins in CDLE and SLE. In CDLE, the reaction product covered a wider area of the superficial dermis and extended deeper down into the dermis. IgG and IgM deposits exhibited an identical fine structural morphology.