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Sensitivity and specificity of the nickel spot (dimethylglyoxime) test
Author(s) -
Thyssen Jacob P.,
Skare Lizbet,
Lundgren Lennart,
Menné Torkil,
Johansen Jeanne D.,
Maibach Howard I.,
Lidén Carola
Publication year - 2010
Publication title -
contact dermatitis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.524
H-Index - 96
eISSN - 1600-0536
pISSN - 0105-1873
DOI - 10.1111/j.1600-0536.2010.01709.x
Subject(s) - dimethylglyoxime , nickel , medicine , sensitivity (control systems) , chemistry , chromatography , organic chemistry , engineering , electronic engineering
Background: The accuracy of the dimethylglyoxime (DMG) nickel spot test has been questioned because of false negative and positive test reactions. The EN 1811, a European standard reference method developed by the European Committee for Standardization (CEN), is fine‐tuned to estimate nickel release around the limit value of the EU Nickel Directive from products intended to come into direct and prolonged skin contact. Because assessments according to EN 1811 are expensive to perform, time consuming, and may destruct the test item, it should be of great value to know the accuracy of the DMG screening test. Objectives: To evaluate the sensitivity and specificity of the DMG test. Methods: DMG spot testing, chemical analysis according to the EN 1811 reference method, and X‐ray fluorescence spectroscopy (XRF) were performed concomitantly on 96 metallic components from earrings recently purchased in San Francisco. Results: The sensitivity of the DMG test was 59.3% and the specificity was 97.5% based on DMG‐test results and nickel release concentrations determined by the EN 1811 reference method. Conclusions: The DMG test has a high specificity but a modest sensitivity. It may serve well for screening purposes. Past exposure studies may have underestimated nickel release from consumer items.