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Variation in the amount of petrolatum preparation applied at patch testing
Author(s) -
Bruze Magnus,
FrickEngfeldt Malin,
Gruvberger Birgitta,
Isaksson Marléne
Publication year - 2007
Publication title -
contact dermatitis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.524
H-Index - 96
eISSN - 1600-0536
pISSN - 0105-1873
DOI - 10.1111/j.1600-0536.2007.00985.x
Subject(s) - patch testing , patch test , test preparation , volume (thermodynamics) , biomedical engineering , nuclear medicine , computer science , medical physics , contact dermatitis , medicine , allergy , physics , quantum mechanics , engineering , immunology , manufacturing engineering
The elicitation of a positive patch test reaction in a given individual depends upon the dose of the sensitizer applied, the patch test technique and the occlusion time. The dose is determined by the concentration and volume/amount of test preparation applied. If the same amount/volume of a test preparation is applied all the time with the same test technique (same area of skin) and occlusion time, it is appropriate to use concentration as a dose parameter. Most contact sensitizers are incorporated in petrolatum (pet.). With pet. as vehicle, it is impossible to repeatedly apply an exact volume/amount. This study was performed to investigate the inter‐ and intra‐individual variation of pet. preparation applied at patch testing by 3 technicians. Weighing demonstrated that the 3 technicians had about the same precision in their pet. application. The investigation demonstrates that there is both an inter‐individual (statistically significant) and intra‐individual variation in the amounts of pet. applied at patch testing for the 3 technicians. Presently, there is no recommendation on what amount of pet. preparation to apply, which merits a decision to be taken based on thorough investigations on the appropriate volumes of pet. preparation to be applied in various patch test systems.