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High‐performance liquid chromatography analysis of rubber allergens in protective gloves used in health care
Author(s) -
Bergendorff Ola,
Persson C.,
Hansson C.
Publication year - 2006
Publication title -
contact dermatitis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.524
H-Index - 96
eISSN - 1600-0536
pISSN - 0105-1873
DOI - 10.1111/j.1600-0536.2006.00912.x
Subject(s) - high performance liquid chromatography , zinc , acetone , chromatography , ketone , chromatography detector , natural rubber , chemistry , organic chemistry
A high‐performance liquid chromatography (HPLC) method developed for analysis of zinc dithiocarbamates was validated and used to perform a survey of disposable medical gloves used in southern Sweden. The gloves were extracted with acetone at room temperature for 10 min by shaking. The extracts were injected into a polyether ether ketone lined HPLC column, and peaks were analysed by a diode‐array detector. The survey shows that of 19 gloves analysed, 10 contained zinc diethyldithiocarbamate (0.070–3.5 mg/g), 3 contained zinc pentamethylenedithiocarbamate (1.0–4.3 mg/g), 4 contained zinc dibutyldithiocarbamate (0.9–1.1 mg/g), and 2 contained 2‐mercaptobenzothiazole (0.005–0.008 mg/g). None of them contained thiurams.