Premium
Dose‐response studies of contact allergens using 3 guinea pig models
Author(s) -
Wahlkvist Helen,
Boman Anders,
Lidean Carola
Publication year - 1999
Publication title -
contact dermatitis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.524
H-Index - 96
eISSN - 1600-0536
pISSN - 0105-1873
DOI - 10.1111/j.1600-0536.1999.tb06130.x
Subject(s) - sensitization , medicine , protocol (science) , immunology , mathematics , pathology , alternative medicine
A multi‐dose‐response induction protocol for the guinea pig maximization test (GPMT), including a statistical computer program, has earlier been developed to improve the power of predictive tests for identification of contact allergens. This dose‐response protocol, with 2 modifications (i.e., increased number of animals in each group and increased number of challenge concentrations) was evaluated in the GPMT, the cumulative contact enhancement test (CCET) and the Freund's complete adjuvant test (FCAT), using potassium dichromate and hydroxycitronellal as model contact allergens. Application of the dose‐response protocol on the CCET and the FCAT resulted in either monotone or non‐monotone curves with significant dose‐response. However, application of the dose‐response protocol on the GPMT gave curves with no significant dose‐response. The protocol makes it possible to obtain an EC 50 value, thus improving the possibility of ranking contact allergens, which is of substantial use for risk assessments. The dose‐response protocol could benefit from a few adjustments: a wider span in the induction doses; change to simultaneous increase in intradermal and topical induction doses to obtain a proper dose‐response for the GPMT; the addition of further challenge concentrations. In addition the computer program should allow calculation of threshold concentration for sensitization and EC 50 value for a non‐monotone curve.