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Lack of antagonism to Ni 2+ and Co 2+ contact allergy from other essential divalent metal ions
Author(s) -
Broeke Leon T.,
Nilsson I Lars G.,
Scheynius Annika,
Wahlberg Jan E.,
Karlberg AnnTherese
Publication year - 1998
Publication title -
contact dermatitis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.524
H-Index - 96
eISSN - 1600-0536
pISSN - 0105-1873
DOI - 10.1111/j.1600-0536.1998.tb05741.x
Subject(s) - antagonism , contact allergy , divalent , divalent metal , metal , chemistry , ion , metal ions in aqueous solution , allergy , inorganic chemistry , contact dermatitis , immunology , medicine , organic chemistry , biochemistry , receptor
The potential antagonistic effects of Ca 2+ , Cu 2+ , Fe 2+ , Mg 2+ , Mn 2+ and Zn 2+ on contact allergy to Co 2+ and Ni 2+ were studied. The immune response was characterized by the Co 2+ or Ni 2+ mediated cellular [methyl‐ 3 H]thymidine uptake in peripheral blood mononuclear cell (PBMC) cultures from 6 subjects contact‐allergic to Co 2+ and Ni 2+ and 6 non‐allergic control individuals. Results from the in vitro experiments were further evaluated with Co 2+ ‐sensitized guinea pigs according to the modified Freund's complete adjuvant test. Ni 2+ and Co 2+ (10–50 μM) significantly increased the lymphocyte proliferation in PBMC cultures from contact‐allergic subjects in comparison with those from control individuals. Pretreatment of the PBMCs with Ca 2+ , Fe 2+ , Mg 2+ (10–100 μM) or Mn 2+ (1–10 μM) did not influence, while Zn 2+ (100 μM) enhanced, and Cu 2+ (5 and 10 μM) markedly reduced the Ni 2+ and Co 2+ mediated cellular [methyl‐ 3 H]thymidine uptake. The inhibition of the Ni 2+ ‐ and Co 2+ ‐induced cell proliferation by Cu 2+ in vitro was probably related to toxicity, since the viability of the cells was significantly reduced by applied combinations of Ni 2+ or Co 2+ with Cu 2+ . Topical pretreatment of Co 2+ ‐sensitized guinea pigs with maximum non‐irritating doses of CuCl 2 · 2H 2 O (0.8%) did not affect the challenge testing to CoCl 2 · 6H 2 O (0.1 and 0.3%). In conclusion, our combined in vitro and in vivo results indicate that Ca 2+ , Cu 2+ , Fe 2+ , Mg 2+ , Mn 2+ and Zn 2+ are not able to antagonise the formation of Ni 2+ and Co 2+ antigens.